Bovine, chicken, equine, goat, guinea pig, human, mammal, mouse, porcine, primate, rabbit, rat, sheep. A novel myelin protein zero transgenic zebrafish designed for rapid readout of in. Myelin basic protein mbp is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Olig1 and sox10 interact synergistically to drive myelin basic. This target gene encodes the protein myelin basic protein in humans and may also be known as golli mbp, prolinerich proteoglycan 2, and myelin a1 protein. A combination of simple genetics, ease of culturing, fast expression and high yields makes it an attractive host while the lack of an. Mbp antibody 10 m59a mouse monoclonal mbp antibody. Antimbp monoclonal antibody is a murine antimaltose binding protein mbp antibody, isotype igg2a. Antibodies, primary antibodies, zfish zebrafish, alphabetical listing of zebrafish antibodies. Hardcopies of the 4th edition of the zebrafish book can be obtained for a nominal fee from the zebrafish international resource center, 5274 university of oregon, eugene, or 97403 usa. For different evaluation purposes we need zebrafish blood. Mbp antibody 34 products in validated antibody database.
For protein purification by immunoprecipitation, the antibodies were crosslinked. So after anaesthesia we can collect the blood by above technique. There are currently no references for myelin basic protein antibody gtx54239. Transcripts of synapsinii a syn2a and myelin basic protein mbp. Diseases associated with mbp include demyelinating disease and secondary progressive multiple sclerosis. Immunohistochemical analysis of a paraffinembedded mouse brain using myelin basic protein d8x4q xp rabbit mab in the presence of control peptide left or antigenspecific peptide right. Neurotoxicity assessment using zebrafish sciencedirect. Our mbp antibodies can be used in a variety of model species.
Functional analysis of novel regulators of cardiac function using zebrafish model duration. Visualization of myelination in gfpatransgenic zebrafish wiley. We appreciate as much detail as possible and references as appropriate. Alternative splicing from the golli and the mbp transcription start sites gives rise to 2 sets of mbp related transcripts and gene products.
To this end, they developed a novel assay based on wholemount immunostaining of motorneurons using specific antibodies for pmns and smns. Among its related pathways are spinal cord injury and glial cell differentiation. Two tsc1 homologs were identified in zebrafish, which we refer to as tsc1a and tsc1b. Rabbit antimyelin basic protein antibody reacts specifically with myelin basic protein of mouse, rat and human. The mbp antibody stains oligodendrocytes and the myelin sheathes around axons. A guide for the laboratory use of zebrafish danio rerio. Generating recombinant protein requires the use of an expression host and e. In this study, we generated a transgenic zebrafish tgmbp.
Alternative splicing of human mbp generates several isoforms which lack amino acids 13, lack aa 240250, andor have a 26 aa insertion following lys192, yielding protein products of 1733 kda. The myelin basic protein antibody has been validated for the following applications. The myelin basic protein antibody from proteintech is a rabbit polyclonal antibody to a recombinant protein of human myelin basic protein. Anatomy of zebrafish cerebellum and screen for mutations affecting. Please use this form to recommend updates to the information in zfin.
We therefore cloned a partial cdna which was most likely to be a zebra fish homologue of mammalian fog1 by the rtpcr method. Select, antibody name, antigen gene name, isotype balbc mice, cellular binding site, recommended dilution, anatomical structure labeled, availability. The peterson lab uses chemical screens in living zebrafish to identify novel compounds that modify. The zebrafish model is a bridge between in vitro assays and mammalian. Due to the covid19 pandemic, resource distribution has been suspended and services may be delayed.
Antimyelin basic protein mbp antibody can be used in western blotting and flow cytometry. Zebrafish tsc1 reveals functional interactions between the cilium. Mbp antibody staining protocol for immunohistochemistry. Antimyelin basic protein recognizes human, mouse, and rat myelin basic protein. Thermo fisher antibodies are validated for applications including western blotting, immunocytochemistry, flow cytometry, and chromatin immunoprecipitation. It is covalently linked to horseradish peroxidase and purified 1,2. To visualize oligodendrocytes and their myelin processes in living zebrafish, we first. To the right of the western blot is a lane from a coomassiestained sdspage gel on which 4 g of purified mbp was loaded. Antimyelin basic protein mbp is produced in rabbit using as immunogen a synthetic peptide corresponding to residues 102116 redntfkdrpsesde of human myelin basic protein geneid 4155. Anti mbp monoclonal antibody datacards the product summary sheet, or data card, includes details for how to use the product, as well as details of its formulation and quality controls. A zebrafish orthologue of mbp is expressed in myelinating, mature oligodendrocytes and schwann cells of zebrafish, suggesting that mbp is a useful marker for myelination brosamle and halpern, 2002. Zebrafish lysate buffer for western blots or good zebrafish antibodies. Zirc monoclonal antibodies zebrafish international. Sections from decalcified whole fish at the level of the optic nerve were stained with the a20 monoclonal antibody a, c, e or the antimbp antibody b, d, f.
A novel model of demyelination and remyelination in a gfp. If you continue, well assume that you are happy to receive all cookies. Anti mbp antibody products anti mbp antibodies are offered by a number of suppliers. Histological sections encompassing the to and labeled with mbp antibody. So, the mbp antibody f6 may need to have higher titer. Myelin basic protein mbp is a protein believed to be important in the process of myelination of nerves in the nervous system.
Myelin basic protein mbp is a major constituent of the myelin sheath of oligodendrocytes and schwann cells in the central nervous system and the peripheral nervous system, respectively. They encode hybrid proteins that have nterminal golli aa sequence linked to mbp aa sequence. The golli mrnas contain 3 exons unique to golli mbp, spliced inframe to 1 or more mbp exons. It is most abundant in hemopoietic system and contains seven exons distributed over 32. Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery. Myelin basic protein mbp antibody phosphosolutions. Mbp is believed to be important in the process of myelination of nerves in the central nervous system cns. As the leading manufacturer of zebrafish antibodies, genetex offers an extensive portfolio of reagents validated for use in this model system.
Verified for use in both western blotting and elisa. Previous reports in zebrafish indicate that the two prion genes, prp1 and prp2. Mbp antibody 2h9 nbp222121 total human brain lysate was separated by sdspage on a 12% gel and transferred to pvdf membrane. For modern pharmacological research zebrafish is a new lab rat. A periaxonal net in the zebrafish central nervous system. Zebrafish danio rerio have emerged as powerful toxicological model. This mbp antibody is generated from rabbits immunized with a klh conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding y203 of human mbp. Compare antizebrafishfish antibody products from leading suppliers on biocompare. Markers and controls zebrafish antibodies sigmaaldrich. May i block the tissue again or only wash and add the new antibody. Zebrafish prion protein prp2 controls collective migration process. Myelin basic protein was tested in an in vitro kinase assay by incubation with lane 1 or without mapk2 lane 2, followed by western blot detection using an mbp phospho thr98 antibody.
Antimbp antibody, anti mbp antibody, myelin basic protein antibody, mbp antibody, myelin basic protein antibody. Immunoprecipitation of sf9 insect cell extract overexpressing mbpakt fusion protein lane 1 or cells without transfection lane 2, using mbp tag 8g1 mouse mab, then western analysis using the same antibody. The optic nerve leaves the eye through the hole of cribriform plate large arrow in a, b. Characterization of myelination in the developing zebrafish. The myelin sheath is a multilayered membrane, unique to the nervous system, that functions as an insulator to greatly increase the velocity of axonal impulse conduction. Recent progress in the zebra fish genome project led us to hypothesize that fog1 may also exist in zebra fish and play important roles in hematopoiesis through interaction with gata1. Antimbp monoclonal antibody hrp conjugated is a murine antimaltose binding protein antibody, isotype igg2a. It is aimed to help labome visitors find the most suited mbp antibody.
A schematic presentation of zebrafish cerebellar neurons and circuits. Improving recombinant protein solubility using the mbp tag e. Please login first to complete purchase quotation request, view custom order reports, or create favorites list. Two novel morpholinos were tested that target the initiation codon mo1. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago eylar et al. Antimyelin basic protein antibody gtx54239 genetex. Use of zebrafish in drug discovery toxicology chemical research. Within aa 13, human mbp shares 79% aa sequence identity with mouse mbp. I suggest running the antiserum over only the mbp affinity column to remove mbp reactivity, but not the fusion protein affinity column. Selfassociation of gata1 enhances transcriptional activity. Myelin basic protein mbp is the major extrinsic membrane protein of central nervous system and has a molecular mass of 21. This avoids binding the antibody that recognizes the protein. Be the first to share your publications with this product.